Expression of chimeric KSAC protein and method of producing soluble proteins by high pressure

摘要

The present invention encompasses vaccines or compositions comprising the chimeric KSAC protein that possesses immunogenic and protective properties, and methods of use including administering to an animal the antigenic KSAC protein thereof to protect animals. The invention also encompasses methods for making and producing the soluble, disaggregated, refolded or active proteins from inclusion bodies produced from prokaryotes or eukaryotes.

主权项

1. A method of producing a soluble protein expressed in prokaryotes or eukaryotes comprising the steps of (i) preparing the inclusion bodies in a buffer comprising no or low concentration of urea to form inclusion body suspension; (ii) subjecting the inclusion body suspension to a target high pressure consisting of a stepwise increase of pressure over a period of time; and (iii) maintaining the high pressure applied to the inclusion bodies for a period of time.