Automated transient image cytometry

摘要

A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each image of an image time sequence in order to localize the transient activity with respect to each cell. Localization enables cell-by-cell analysis of properties of the transient activity.

主权项

1. An instrument for obtaining a time-series sequence of magnified images of transient activity in cells, comprising:
an adjustable mechanical stage to position a well of multi-well plate; means for applying a stimulation signal in the well; a microscope objective with a field of view containing the well; an autofocus control mechanization operative to adjust the mechanical stage vertically to a best focus position where the contents of the well are best focused by the objective; and, a camera optically coupled to the microscope objective and operative to acquire a time-series sequence of magnified images of the contents.