Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid

摘要

Provided is a novel hyaluronan synthase (HAS2), isolated nucleic acids encoding it, and expression vectors which express the novel HAS2. Also provided are cell cultures which contain cells which harbor the expression vectors, methods of using the cell cultures to produce high molecular weight hyaluronic acid, and cell culture media that contains the high molecular weight hyaluronic acid.

主权项

1. A population of eukaryotic cells comprising a recombinant expression vector encoding a protein, wherein the protein comprises an amino acid sequence having at least 98% amino acid homology to the sequence of SEQ ID NO:1, wherein the amino acid sequence comprises a serine at amino acid positions 178 and 301 relative to the sequence of SEQ ID NO: 1.