New method for inducing dopamine-producing neural precursor cells

摘要

Provided is a method for manufacturing dopamine-producing neural precursor cells from pluripotent stem cells, the method comprising: (i) a step in which pluripotent stem cells are cultured adherently on an extracellular matrix in a culture solution containing a reagent selected from the group consisting of BMP inhibitors, TGF&bgr; inhibitors, SHH signal stimulants, and FGF8 and GSK3&bgr; inhibitors; (ii) a step in which a substance than binds to Corin and/or a substance that binds to Lrtm1 is used to collect Corin and/or Lrtm1 positive cells from the cells obtained in step (i); and (iii) a step in which the cells obtained in step (ii) are cultured in suspension in a culture solution containing a neurotrophic factor.