摘要 |
PROBLEM TO BE SOLVED: To reduce nonspecific reaction in nucleic acid amplification reaction.SOLUTION: The present invention provides a method for detecting RNA included in a sample, wherein, in the presence of a molecule comprising at least one phosphoric acid excluding deoxynucleotide triphosphate, a heat-resistant DNA polymerase and a reverse transcriptase are used for nucleic acid amplification by RT-PCR.SELECTED DRAWING: None |