发明名称 |
PRODUCTION OF DIRHAMNOSE-LIPID IN RECOMBINANT NONPATHOGENIC BACTERIUM PSEUDOMONAS CHLORORAPHIS |
摘要 |
Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C12:1-C10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C12-C10) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R1L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R1L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R2L) is described. Characterization of R1L and R2L produced by the recombinant P. chlororaphis NRRL B-30761 is also described. |
申请公布号 |
US2016102330(A1) |
申请公布日期 |
2016.04.14 |
申请号 |
US201414892793 |
申请日期 |
2014.06.03 |
申请人 |
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE |
发明人 |
Solaiman Daniel;ASHBY Richard D.;ZERKOWSKI Jonathan A.;GUNTHER Nereus W. |
分类号 |
C12P19/44;C12P7/64;C12N9/10 |
主分类号 |
C12P19/44 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Washington DC US |