PRODUCTION OF FATTY ACID ESTERS

摘要

PROBLEM TO BE SOLVED: To provide methods for producing fatty acid esters by culturing a microorganism genetically modified to produce a fatty acid and to express a wax ester synthase in the presence of an exogenous alcohol.SOLUTION: The invention provides a cell comprising a first genetic mutation and a second genetic mutation, where the first genetic mutation enables production of a fatty acid of at least 5 carbon atoms and/or acyl-Coenzyme A (CoA) thereof by increasing the enzymatic activity of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathways, and the second genetic mutation increases the activity of a wax ester synthase having at least 50% sequence identity to a polypeptide of SEQ ID NOs: 1-8 and a combination thereof or to a functional fragment of any polypeptide that catalyzes conversion of a fatty acid and/or acyl-CoA thereof to a fatty acid ester.SELECTED DRAWING: None