METHOD AND USE OF NUCLEIC ACID ISOTHERMAL AMPLIFICATION VIA POLYMERASE SPIRAL REACTION

摘要

Disclosed are a method and use of nucleic acid isothermal amplification via a polymerase spiral reaction. Also provided is a primer comprising a specific primer and n accelerating primers A; the specific primer consists of a forward primer FP and a backward primer BP; the forward primer FP sequentially consists of an N region and an F region from a 5' end to a 3' end, the F region being identical to or complementary with an Fc region adjacent to the 3' end in a target sequence; the backward primer BP sequentially consists of an N' region and a B region from a 5' end to a 3' end, the B region being identical to or complementary with a Bc region adjacent to the 5' end in a target sequence; and the N' region is a reverse non-complementary sequence of the N region. The nucleic acid isothermal amplification method of the present invention completes nucleic acid amplification with only one pair of primers, employs a self-spiraling amplification method, and has a high amplification efficiency.