| 摘要 |
A method is provided for determining a sample concentration of target entities in a sample, for example, determining a concentration of target antigens or antibodies in a blood sample or other biological sample. The method comprises obtaining assay data comprising data points of respective local measurements indicative of a local concentration of target entities immobilised at each of a plurality of assay areas of an assay assembly from an assay using the assay assembly. The assay areas are connected in series such that a sample flowing through the assay assembly flows past each assay area in sequence. Each assay area comprises a plurality of probe entities immobilized at a surface of the assay area, the probe entities being arranged to bind to the target entities in the sample, such that the concentration of the target entities is depleted as the sample flows from one of the assay areas to the next. The assay data is modeled with a parameterized function of the local measurements against a quantity indicative of the position of the respective assay areas in the sequence, wherein one or more of the parameters are dependent on the sample concentration. A value indicative of the sample concentration is determined based on at least one of the one or more parameters. |
