摘要 |
The invention relates to a microscope (10) comprising an automatic focusing system (11) for carrying out a focusing step, with a first image sensor (14a) which is arranged in a first coupled out beam path (12a) and which is used to capture a first image (16a) and a second image sensor (14b) which is arranged in a second coupled out beam path (12b) and which is used to capture a second image (16b). The automatic focusing system (11) is designed in such a manner that, based on contrast values of the first image (16a) captured by the first image sensor (14a) and the second image (16b) captured by the second image sensor (14b) a contrast difference is determined, and based on the determined contrast difference, a relative position of the focus plane (20) to the object plant (22) is adjusted, the first and the second images (16a, 1bb) respectively comprising image information provided by the respective first and second image sensor (14a, 14b) designed as surface sensors. |