STRAND-INVASION BASED DNA AMPLIFICATION METHOD

摘要

A method for detecting toxigenic Clostridium difficile (C. difficile) by strand-invasion based DNA amplification is provided, together with oligonucleotides, compositions and kits suitable for use in this method.

主权项

1. A method for detecting a target nucleic acid sequence of toxigenic C. difficile in a sample, said method comprising contacting said sample with at least one upstream primer, at least one downstream primer and at least one strand invasion oligonucleotide under conditions promoting amplification of said target nucleic acid sequence,
wherein each said primer and said strand invasion oligonucleotide comprises a region complementary to said target nucleic acid sequence; and wherein said strand invasion oligonucleotide renders at least a portion of the target nucleic acid sequence single-stranded to allow the binding of said upstream primer and a downstream primer.