发明名称 |
SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE AND METHODS OF PRODUCING SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE |
摘要 |
Embodiments of the claimed invention relate to sex sorted sperm having an improved dose response which may be produced by the steps of extending a sperm sample with a buffering holding media and adjusting the concentration of the extended sperm sample to a target concentration range. The extended sperm may then be stained with a DNA selective dye and sex sorted with a flow cytometer into a catch fluid. During the extending and sorting of the sperm sample, the pH may be maintained at a target pH. Additionally, at least one of the buffering holding media, the DNA selective dye and the catch fluid may be supplemented with at least one antioxidant. |
申请公布号 |
AU2015230805(A1) |
申请公布日期 |
2016.04.14 |
申请号 |
AU20150230805 |
申请日期 |
2015.09.25 |
申请人 |
INGURAN, LLC |
发明人 |
VISHWANATH, RAMAKRISHNAN;EVANS, KENNETH MICHAEL;GILLIGAN, THOMAS B.;MORENO, JUAN;LENZ, RICHARD;GONZALEZ-MARIN, CLARA |
分类号 |
C12N5/076 |
主分类号 |
C12N5/076 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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