CLONATION, EXPRESSION AND USE OF ACID LYSOPHOSPHOLIPASES

摘要

DNA sequence (I) coding for an Aspergillus fumigatus lysophospholipase (II) is new. DNA sequence (I) having SEQ ID NO:1 (defined sequence of 2507 nucleotides given in the specification) and coding for a lysophospholipase (II) with SEQ ID NO:2 (defined sequence of 611 amino acids given in the specification) is new. Independent claims are also included for: (1) expression construct comprising (I) linked to regulatory sequences for expression in a host cell; (2) recombinant host cell transformed with the expression construct; (3) plasmid B6Sma35 (DSM 18370); (4) phospholipase encoded by the coding portion (especially nucleotides 580-2367) of (I); (5) producing (II) by culturing a host cell as above.