发明名称 |
COMPOSITIONS AND METHODS FOR TREATING PERIPHERAL ARTERIAL DISEASE |
摘要 |
The present application discloses roles for miR-93 in treating hypoxia and ischemia. Endothelial cells (HUVEC) and myocytes (C2C12) expressed miR-93 and up-regulated miR-93 in response to hypoxia and serum starvation. Over-expression of miR-93 in HUVECs promoted cell proliferation, prevented hypoxia-induced apoptosis, and enhanced endothelial cell tube formation. miR-93 knockdown in HUVECs resulted in increased hypoxia-induced apoptosis and decreased tube formation. Over-expression or knockdown of miR-93 in myocytes resulted in reduced or increased hypoxia-induced apoptosis, respectively. Down-regulation of miR-93 in C57BL/6 mice with antagomiR resulted in attenuated perfusion recovery (% non-ischemic leg at day-21: Scramble 85.22.9 vs. AntagomiR-93 67.96). Over-expression of miR-93 in BALB/C mice improved perfusion recovery (% non-ischemic leg at day 21: PremiR-93 757.5 vs. Scramble 59.62.5). The present invention encompasses the use of miR-93 and regulation of miR-93 to treat and prevent hypoxia, ischemia, and other injuries, diseases, disorders, and conditions associated with ischemia. |
申请公布号 |
EP2885008(A4) |
申请公布日期 |
2016.04.13 |
申请号 |
EP20130829411 |
申请日期 |
2013.08.15 |
申请人 |
UNIVERSITY OF VIRGINIA PATENT FOUNDATION;ANNEX, BRIAN H.;FARBER, CHARLES R.;HAZARIKA, SUROVI |
发明人 |
ANNEX, BRIAN, H.;FARBER, CHARLES, R.;HAZARIKA, SUROVI |
分类号 |
A61K48/00;A61P7/00;A61P7/06 |
主分类号 |
A61K48/00 |
代理机构 |
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