发明名称 |
EXTRACELLULAR DITERPENE PRODUCTION |
摘要 |
The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium. |
申请公布号 |
EP3004365(A1) |
申请公布日期 |
2016.04.13 |
申请号 |
EP20140727820 |
申请日期 |
2014.06.02 |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
BOER, VIKTOR MARIUS;BROERS, NICOLETTE, JASMIJN;LAWRENCE, ADAM, G. |
分类号 |
C12P15/00;A23L2/60;A23L27/30;C12N9/02;C12N9/10;C12N9/88;C12N9/90;C12N15/52;C12P19/56 |
主分类号 |
C12P15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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