Solid phase for mixed-mode chromatographic purification of proteins

摘要

Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.

主权项

1. A method for purifying a non-antibody protein from a source solution, said method comprising:
(a) contacting said source solution at a pH of 4.0 to 6.0 with a mixed-mode chromatography medium comprising a ligand coupled to a solid support, said ligand comprising benzamidoacetic acid, said solid support having pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter, and said ligand coupled to said solid support at a phenyl ring through a chain of one to three atoms, to bind said non-antibody protein in said source solution to said solid support through said ligand; and (b) eluting said non-antibody protein so bound from said solid support.