Mannanase produced from <i>Cellulosimicrobium </i>sp. strain HY-13

摘要

The present invention relates to a mannanase produced from Celluosimicrobium sp. strain HY-13 and more particularly to a highly active novel mannanase produced from Cellulosimicrobium sp. strain HY-13 as separated from an invertebrate. The mannanase produced from the said Cellulosimicrobium sp. strain HY-13 can be used in the decomposition of a mannan found in hemicellulose contained in grains and plants and in enhancing the utility value of a hydrolysate, which uses the decomposed mannan, for the transformation of biomass or as an animal feed material.

主权项

1. A method for producing a mannanase comprising the following steps:
1) culturing Celluosimicrobium sp. strain HY-13 deposited under the Accession Number of KCTC 11302BP in a medium containing mannan, and obtaining supernatant by centrifuging thereof; 2) inducing precipitation of water-soluble proteins by adding a precipitant to the supernatant of step 1); 3) obtaining a crude enzyme solution by dialyzing the precipitate of step 2) after eliminating insoluble precipitate from the same; and 4) purifying the mannanase from the crude enzyme solution of step 3).