发明名称 Modulation of meiotic recombination
摘要 The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution. Methods of plant and animal breeding are also provided that utilize the modulation of meiotic homologous recombination.
申请公布号 US9309525(B2) 申请公布日期 2016.04.12
申请号 US201414216907 申请日期 2014.03.17
申请人 Her Majesty the Queen in Right of Canada, as Represented by the Minister of Agriculture and Agri-Food 发明人 Rozwadowski Kevin L.;Lydiate Derek J.
分类号 C12N15/63;C12N15/81;C12N15/82;C12N15/52;C12N15/873;C12N15/90 主分类号 C12N15/63
代理机构 Knobbe, Martens, Olson & Bear, LLP 代理人 Knobbe, Martens, Olson & Bear, LLP
主权项 1. A eukaryotic cell, wherein the cell is a yeast cell or a plant cell, the cell comprising a nucleic acid encoding a recombinant protein that is homologous to an endogenous protein of the yeast cell or the plant cell, wherein the recombinant protein is a DMC1 protein or a RAD51 protein, wherein the nucleic acid is operably linked to a promoter, wherein expression of the recombinant protein in the cell during meiosis increases the frequency of homologous non-sister chromatid exchange during meiosis compared to a eukaryotic cell that does not comprise the nucleic acid.
地址 Saskatchewan CA