Methods and compositions for delivering interleukin-1 receptor antagonist

摘要

Methods and compositions generating and using an interleukin-1 receptor antagonist (IL-1ra)-rich solution. Methods for generating and isolating interleukin-1 receptor antagonist include incubating adipose tissue and/or adipocytes with polyacrylamide beads to produce interleukin-1 receptor antagonist. The interleukin-1 receptor antagonist is isolated from the polyacrylamide beads to obtain the solution rich in interleukin-1 receptor antagonist. Methods for treating a site of inflammation in a patient include administering to the site of inflammation the solution rich in interleukin-1 receptor antagonist.

主权项

1. A method of treating a site of inflammation in a patient comprising:
(a) contacting a liquid volume comprising isolated adipose tissue with polyacrylamide beads, wherein the polyacrylamide beads activate the isolated adipose tissue to produce interleukin-1 receptor antagonist to form a solution rich in interleukin-1 receptor antagonist; (b) separating the solution rich in interleukin-1 receptor antagonist from the isolated adipocytes and the polyacrylamide beads; and (c) administering the solution rich in interleukin-1 receptor antagonist to the site of inflammation in the patient.