| 摘要 |
PROBLEM TO BE SOLVED: To enable the cumulative number of data integration that is suitable for identifying a target peptide to be automatically set without excess or deficiency.SOLUTION: MS/MS analysis for a standard peptide of a prescribed concentration that is added to a test sample is executed repeated as many times as the cumulative number of data integration, the profile data thereby obtained is added up, and an MS/MS spectrum is found (S2). The peptide is identified by performing a database search based on the MS/MS spectrum, and the score or expected value indicating the reliability of identification is calculated (S3). When the identification fails or the score or expected value does not reach a prescribed threshold although the identification is successful (No in S4), the cumulative number of data integration is increased (S5) and the MS/MS analysis for the standard peptide is additionally executed as many times as increased, the results of these executions are added to the integration profile data, and the MS/MS spectrum is found. This way, the cumulative number of data integration is increased until determination of Yes is made in S4, and the cumulative number of data integration for the target peptide is determined and the MS/MS analysis for the target peptide is carried out(S6).SELECTED DRAWING: Figure 2 |
