INTERNALIZATION OF PROTEINS INTO HOLLOWED GOLD NANOSTRUCTURES

摘要

Provided is a gold nanocage with pores, charged ligand molecules covalently bound to internal surfaces of the gold nanocage, and payload molecules electrostatically adsorbed onto said charged ligand molecules, wherein a pore diameter is between 1.2 and 20 times a gyration radius of the payload molecule. Also provided is a method for making a nanoparticle, including using polyvinylpyrrolidone as a capping agent in a galvanic replacement reaction to convert a silver nanocube into a gold nanocage having pores, replacing the polyvinylpyrrolidone on internal surfaces with charged ligand molecules, and electrostatically adsorbing payload molecules onto the charged ligand molecules, with a pore diameter less than twenty times a gyration radius of the payload molecule. Also provided is a method of delivering a pharmacological agent to a mammalian cell, including contacting the cell with a gold nanocage having pores, ligand molecules bound to internal surfaces, and pharmacological agent adsorbed onto ligand molecules.

主权项

1. A nanoparticle comprising:
a gold nanocage comprising pores; charged ligand molecules covalently bound to one or more surfaces of the gold nanocage, wherein at least some charged ligand molecules are covalently bound to internal surfaces of the gold nanocage; and payload molecules electrostatically adsorbed onto said charged ligand molecules covalently bound to internal surfaces of the gold nanocage, wherein a diameter of one or more pores in the gold nanocage is between 1.2 and 20 times a gyration radius of the payload molecule.