发明名称 |
METHOD FOR PREPARING NK CELLS |
摘要 |
The object of the present invention is to develop a technique in which NK cells having a high cytotoxic activity can be prepared with high purity from hematopoietic precursor cells without using a serum or feeder cells of an animal.;A method for expanding NK cells includes the steps of expanding hematopoietic precursor cells under a single culturing condition using a medium supplemented with IL-15, SCF, IL-7 and Flt3L, and differentially inducing the cells obtained in the expanding step into NK cells under a culturing condition using a medium supplemented with IL-2. A pharmaceutical composition contains the NK cells prepared by the method for expanding NK cells of the present invention. The pharmaceutical composition of the present invention is used for treating an infectious disease and/or a cancer. |
申请公布号 |
US2016097035(A1) |
申请公布日期 |
2016.04.07 |
申请号 |
US201414892248 |
申请日期 |
2014.05.05 |
申请人 |
KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ;TELLA, INC. |
发明人 |
Yonemitsu Yoshikazu;Saito Satoru;Harada Yui;Yazaki Yuichiro;Ishidao Takefumi |
分类号 |
C12N5/0783;A61K35/17 |
主分类号 |
C12N5/0783 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Fukuoka JP |