RECOMBINANT ESCHERICHIA COLI FOR PRODUCING SUCCINIC ACID AND APPLICATION THEREOF

摘要

The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.

主权项

1. A recombinant E. coli, comprising the modifications of:
(1) inhibited expression of the gene(s) involved in phosphoenolpyruvate:sugar phosphotransferase system (PTS), and/or inhibited activities of the protein(s) encoded by the gene(s) involved in phosphoenolpyruvate:sugar phosphotransferase system (PTS), (2) inhibited expression of pflB and/or adhE genes, and/or inhibited activities of the proteins encoded by pflB and/or adhE genes, (3) inhibited expression of ldhA gene, and/or inhibited activity of the protein encoded by ldhA gene, and (4) enhanced expression of galP gene and/or exogenous glf gene, and/or enhanced activities of the proteins encoded by galP gene and/or exogenous glf gene; wherein said E. coli further comprises one or more of the modifications of (a) enhanced expression of the gene(s) involved in pentose phosphate pathway (PPP), and/or enhanced activities of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP); and (b) enhanced expression of sthA gene, and/or enhanced activity of the protein encoded by sthA gene.