MODIFIED MICROORGANISMS AND METHODS FOR PRODUCTION OF USEFUL PRODUCTS

摘要

The invention provides non-naturally occurring microbial organisms and related methods, processes and materials wherein the microbial organisms includes a genetic modification which enhances production of 3-hydroxybutanal or a downstream product of 3- hydroxybutanal such as 1,3-butanediol from endogenous central metabolic intermediates such as acetyl CoA or pyruvate which are converted to acetaldehyde, whereby two molecules of acetaldehyde are condensed to form said 3-hydroxybutanal using an aldolase capable of accepting acetaldehyde as both the acceptor and donor in an aldol condensation. The aldolase may be a deoxyribose phosphate aldolase type enzyme, and is typically introduced into the organisms. The invention provides for energetically favorable pathways for production of 3-hydroxybutanal or downstream products thereof.