| 摘要 |
The present invention relates to a chromatographic process, which process comprises contacting a composition with a hydrophilic stationary phase, which composition comprises: a)one or more analytes; b)water; c)an organic solvent; and d)an ion-pairing reagent; wherein the concentration of the ion-pairing reagent in the composition is less than or equal to 50 mM. Also described is a hydrophilic interaction liquid chromatographic process, which process comprises contacting a hydrophilic stationary phase with a composition comprising one or more analytes and an ion-pairing reagent, wherein the concentration of the ion-pairing reagent is less than or equal to 50 mM. The invention also relates to a chromatographic process, which process comprises (i) contacting a composition with a hydrophilic stationary phase, which composition comprises: a) one or more analytes; b) water; c) an organic solvent; and d) an ion-pairing reagent; and (ii) a step of analysing the composition which has been contacted with the hydrophilic stationary phase by mass spectrometry, evaporative light scattering detection(ELSD), charged aerosol detection (CAD), fluorescence detection, refractive index detection, chemiluminescence detection, optical rotation detection, or electrochemical detection. Uses and an apparatus are also described. |
