METHODS FOR SEQUENCE-DIRECTED MOLECULAR BREEDING

摘要

The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.

主权项

1. A method of plant breeding comprising:
providing a plurality of plant DNA samples, wherein at least one of the plant DNA samples comprises a polymorphism; amplifying one or more selected loci in the genome of two or more of the plant DNA samples, wherein each of said selected loci is amplified with at least one primer comprising at least one DNA tag and at least one allele-specific tag; combining at least two of the amplified DNA samples to create a pool of amplified DNA samples; sequencing the allele-specific tags in the pool of amplified DNA samples; comparing the sequence information from the allele-specific tags to a known library of allele-specific tags to identify the presence or absence of the polymorphism in individual DNA samples, wherein the individual DNA samples are identified by the DNA tags; and selecting one or more plants for use in a plant breeding program based upon the presence or absence of the polymorphism in the plant DNA samples.