摘要 |
[Object] To provide a means which is suitable for mass culture of adherent cells and which allows easy and safe collection of the cultured adherent cells. [Solution] A cell culture kit 10 has a culture bag 11 which is formed by shaping at least one sheet having flexibility, such a level of hydrophilicity that the culture of adherent cells can be achieved, and permeability into a bag shape and which is filled with a culture medium; a dissociation solution bag 12 which is filled with a dissociation solution containing a metal chelating agent or ornithine or a derivative thereof, and a solution sending circuit 13 which connects the culture bag 11 and the dissociation solution bag 12 to each other and can send the dissociation solution from the dissociation solution bag 12 to the culture bag 11. |