Direct detection of unamplified hepatitis C virus RNA using unmodified gold nanoparticles

摘要

A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to HCV RNA. A method for detecting hepatitis C virus comprising contacting a sample suspected of containing hepatitis C virus with a polynucleotide that binds to hepatitis C virus RNA and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting hepatitis C virus in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red).

主权项

1. A method for detecting hepatitis C virus (HCV) comprising:
contacting unamplified RNA obtained from a patient suspected of being infected with HCV with a single-stranded DNA oligotargeter that ranges in length from 19 to 31 contiguous bases and that binds to HCV RNA, for a time and under conditions sufficient for the unamplified HCV RNA and oligotargeter to hybridize, contacting a mixture of the HCV RNA and oligotargeter DNA with non-functionalized gold nanoparticles, and detecting HCV RNA in the sample when the gold nanoparticles aggregate, wherein aggregated gold nanoparticles exhibit a blue color and non-aggregated gold nanoparticles exhibit a red color.