Immobilization of enzymes

摘要

The present invention relates to the immobilization of enzymes by adsorbing enzymes, a polyfunctional amine and a cross-linking agent onto a particulate porous carrier in a mixer apparatus or in a fluid bed apparatus.

主权项

1. A process for producing an immobilized enzyme preparation comprising the following steps:
a) preparing a first liquid medium by adding a solution of a polyfunctional amine to a liquid comprising an enzyme; b) preparing a second liquid medium comprising a cross-linking agent capable of reacting with the polyfunctional amine and the enzyme; and c) introducing the first liquid medium onto a particulate porous carrier and then introducing the second liquid medium onto the particulate porous carrier, and wherein the amount of first liquid medium and second liquid medium introduced onto the particular porous carrier does not result in the exceeding of the adsorption capacity of the particulate porous carrier for liquid medium, thereby producing an immobilized enzyme preparation.