Preparation of nanometric arrays of biomolecules on oligo- or poly(ethylene glycol) films on silicon surfaces

摘要

The present invention is generally directed to nanometric biomolecular arrays and to a novel approaches for the preparation of such nanoarrays, based on binding of biomolecules, such as avidin, to templates generated by lithographically-anodizing biocompatible ultrathin films on silicon substrates using AFM anodization lithography. The present invention is also directed to methods of using such arrays.

主权项

1. A method comprising the steps of:
a) contacting OEG-terminated alkenes with a hydrogen-terminated Si surface to form a contacted surface; wherein the OEG-terminated alkenes comprise α-heptyl-(ethylene glycol) methyl ω-undecenyl ether, wherein the Si surface is selected from the group consisting of Si(100), Si(111), and combinations thereof; b) photolyzing the contacted surface to effect Si—C bonding between the OEG-terminated alkenes and the Si surface and form a OEG-coated Si surface comprising a monolayer of OEG bound to the Si surface through Si—C bonds, wherein the monolayer of OEG resists non-specific adsorption of biomolecules with greater than 99% reduction in adsorption with respect to the Si surface; c) lithographically anodizing the head groups of the OEG monolayer of the OEG-coated Si surface via AFM anodization lithography to yield a nanolithographically-patterned OEG-coated Si surface, wherein the nanolithographically-patterned regions are further etched to form nanoholes, wherein the nanoholes comprise regions with enhanced associability toward biomolecules, wherein the nanolithographically-patterned regions resist denaturing of biomolecules; and d) depositing at least one type of biomolecule in the nanolithographically-patterned regions of enhanced associability to form a nanometric biomolecular array.