发明名称 |
METODO DE ACTIVACION QUIMICA SUPERFICIAL DE UN SOPORTE SOLIDO EN BASE SILICIO MEDIANTE ANCLAJE COVALENTE DIRECTO DE AL MENOS UNA BIOMOLECULA DE ACIDO NUCLEICO. |
摘要 |
The invention relates to a method for chemical surface activation, by a thiol-ene (TEC) or thiol-yne (TYC) reaction, of a solid silicon-based support by means of direct covalent anchoring of at least one biomolecule which is a sequence of DNA or RNA oligonucleotides, where a) the surface of the support is functionalised with alkene or alkyne groups and the biomolecule has a terminal thiol group, and/or b) the surface of the support is functionalised with thiol groups and the biomolecule has a terminal alkene or alkyne group. Said method comprises the steps of: depositing the sequence of oligonucleotides on the surface of the support by means of contactless or contact printing techniques; and anchoring the sequence of oligonucleotides to the surface of the support by radical addition by means of a TEC or TYC reaction caused by photoirradiation with near-visible UV light over an interval of between 5 minutes and 3 hours, without requiring the use of crosslinkers and/or catalysts. |
申请公布号 |
ES2537097(B1) |
申请公布日期 |
2016.04.05 |
申请号 |
ES20130031587 |
申请日期 |
2013.10.30 |
申请人 |
UNIVERSITAT POLITÈCNICA DE VALÈNCIA;CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC);CENTRO DE INVESTIGACION BIOMEDICA EN RED EN BIOINGENIERIA BIOMATERIALES Y NANOMEDICINA (CIBER-BBN) |
发明人 |
BANULS POLO, MARIA JOSE;MAQUIEIRA CATALA, ANGEL;ERITJA CASADELLA, RAMON;ESCORIHUELA FUENTES, JORGE;GRIJALVO TORRIJO, SANTIAGO;PUCHADES PLA, ROSA |
分类号 |
C07H21/00;B01J19/12;C07F7/08;G01N1/28;G01N33/50 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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