| 发明名称 |
Compositions and methods for RNA hybridization applications |
| 摘要 |
The invention provides methods and compositions for hybridizing at least one molecule to an RNA target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in RNA hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to enable hybridization to RNA. |
| 申请公布号 |
US9303287(B2) |
申请公布日期 |
2016.04.05 |
| 申请号 |
US200913203137 |
申请日期 |
2009.12.02 |
| 申请人 |
DAKO Denmark A/S |
发明人 |
Matthiesen Steen Hauge |
| 分类号 |
C12Q1/68;C07H21/00 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A composition comprising at least one polar aprotic solvent in an amount effective to enable hybridization of a nucleic acid sequence to an RNA sequence within a cell in a sample having a preserved cell morphology, and at least 10% dextran sulfate, wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO). |
| 地址 |
Glostrup DK |
