Intein-modified enzymes, their production and industrial application

摘要

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

主权项

1. A mutant intein comprising an amino acid sequence having 100% identity to a sequence of the intein sequence within SEQ ID NO: 1701, wherein the mutant intein is inducible to cause splicing of a protein in which the intein is inserted upon exposure to a temperature of 50° C. or greater.