| 发明名称 | Alpha-amylase variants | ||
| 摘要 | A variant polypeptide having alpha-amylase activity is disclosed. The variant polypeptide has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue with reference to SEQ ID NO: 2. The variant polypeptide has one or more altered properties as compared with a reference polypeptide having alpha-amylase activity. Such a variant polypeptide may be used in the preparation of a baked product. | ||
| 申请公布号 | US9301533(B2) | 申请公布日期 | 2016.04.05 |
| 申请号 | US201313842344 | 申请日期 | 2013.03.15 |
| 申请人 | DSM IP ASSETS B.V. | 发明人 | De Jong Rene Marcel;Abbas Hanna Elisabet |
| 分类号 | C12N9/28;C07H21/04;A21D8/04 | 主分类号 | C12N9/28 |
| 代理机构 | MMWV IP, LLC. | 代理人 | MMWV IP, LLC. |
| 主权项 | 1. A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with an alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acid positions 13, 70, 75, 129, 133, 254, 281, 282, and 358, said positions being defined with reference to SEQ ID NO: 2, wherein the variant is at least 90% identical to SEQ ID NO: 2, and wherein the variant has at least one altered property as compared with a reference polypeptide having alpha-amylase activity, wherein the at least one altered property is increased sucrose tolerance. | ||
| 地址 | Herleen NL | ||