| 主权项 |
1. A method for direct detection of carbapenem-resistant bacteria in a biological sample that comprises a mixture of different bacterial strains, said method comprising the following successive steps:
a) inoculating a culture medium with said sample, wherein the culture medium comprises a single antibiotic and at least one chromogenic agent wherein said chromogenic agent is sensitive to the activity of at least one enzyme selected in the group consisting of β-D-glucuronidase, β-galactosidase, β-D-glucosidase, and a deaminase, and wherein the antibiotic is meropenem at a concentration between 0.25 and 2 mg/L, b) incubating said culture medium under conditions allowing the growth of carbapenem-resistant bacteria, and c) detecting colonies formed on said culture medium corresponding to carbapenem-resistant bacteria, wherein said method does not include a preliminary isolation step of the different bacterial strains present in said biological sample; wherein said chromogenic agent is hydrolysed into a chromophore selected from a group consisting of O-nitrophenyl, P-nitrophenyl, ChloroNitrophenyl, Hydroxyphenyl, Nitroanilide, Phenolphthalein, Thymophthalein, Hydroxyquinoline, Cyclohexenoesculetine, Dihydroxyflavone, Catechol, Resazurin, Resofurin, VBzTM, VLM, VLPr, VQM, Indoxyl, 5-bromo-4-chloro-3-indoxyl, 5-bromo-6-chloro-3-indoxyl, 6-chloro-3-indoxyl, 6-fluoro-3-indoxyl, 5-lodo-3-indoxyl, and N-Methylindoxyl; and, said carbapenem-resistant bacteria are selected from the group consisting of the following genera: Pseudomonas, Burkholderia, Acinetobacter, Proteus, Serratia, Enterobacter, Klebsiella and Escherischia. |
