| 摘要 |
Disclosed is an assay method for determining the potency of anti-MIF antibodies, wherein a cell migration is performed, wherein the assay method comprises the following steps: providing cells which are capable of migration In a first part of a device, wherein the cells comprise MIF, adding the anti-(ox)MIF antibody to be determined, to a second part of the device, which Is configured to be in a connection with the first part of the device which allows cell migration and diffusion, and calculating inhibition of migration, wherein the device comprises an upper and a lower chamber, which are connected via a separating membrane with pores and in that the cells are added to the upper chamber and in that the antibodies are added to the lower chamber and wherein no (ox)MIF is added to the device. |
