| 摘要 |
Discloses a composition comprising: (i) a first molecular complex comprising: a first catalytic nucleic acid enzyme; or a catalytic portion thereof; or a nucleotide sequence complementary to the enzyme or the catalytic portion thereof; hybridised to a first blocker oligonucleotide (BL) by complementary base pairing, wherein at least one: nucleotide, and catalytic core nucleotide of the first enzyme or the catalytic portion thereof, or nucleotides of the sequence that is complementary to a catalytic core is hybridised to the first BL, and the first catalytic nucleic acid enzyme is not an aptazyme; (ii) a nucleic acid substrate capable of catalytic modification by the first catalytic nucleic acid. Also disclosed are kits and methods of detecting molecules using related molecules. |
