| 摘要 |
Disclosed is a method for providing a composite image of a single biological sample. The method comprises the steps of: (1) generating a first image of the biological sample, (2) generating a second image of the biological sample and (3) generating a composite image that provides the relative location of both the target protein and the target nucleic acid. The step of generating a first image of the biological sample comprises the sub-steps of: a. contacting the sample on a solid support with a first binder for a target protein; b. staining the sample with a fluorescent marker that provides morphological information; c. detecting, by fluorescence, signals from the first binder and the fluorescent marker; d. generating the first image of at least part of the sample from the detected fluorescent signals. The step of generating a second image of the biological sample comprises the sub-steps of a. contacting the same sample from step (1) with a probe for each of at least one target nucleic acid sequence thus hybridizing the probes with the target nucleic acid sequence; b. optionally, staining the sample with the fluorescent marker; c. detecting, by fluorescence, signals from the probes for each of the target nucleic acid sequences and the fluorescent marker; and d. generating the second image of at least part of the sample from the detected fluorescent signal. |
