METHOD FOR IN VITRO DIRECTIONAL DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO CARDIAC MUSCLE CELLS

摘要

Provided is a method for in vitro directional differentiation of pluripotent stem cells into cardiac muscle cells under three-dimensional suspension conditions. Also provided is a culture medium for establishing in vitro induced cardiac muscle cell differentiation under three-dimensional suspension conditions. The culture medium comprises a differentiation culture medium for induced differentiation of pluripotent stem cells into mesoderm precursor cells, a differentiation culture medium for differentiation of mesoderm precursor cells into cardiac muscle cells, and a culture medium for long-term holding of cardiac muscle cells.