Engineered Cells Expressing Multiple Immunomodulators and Uses Thereof

摘要

This invention relates to the field of therapeutics. Most specifically invention provides methods of generating in vitro engineered immune cells conditionally expressing interleukin-12 (IL-12) and one or more immunomodulators under the control of a gene expression modulation system in the presence of activating ligand and uses for therapeutic purposes in animals.

主权项

1. A vector for conditionally expressing proteins having the functions of one or more immunomodulators comprising a polynucleotide encoding a gene switch, wherein said polynucleotide comprises (1) at least one transcription factor sequence which is operably linked to a promoter, wherein said at least one transcription factor sequence encodes a ligand-dependent transcription factor, and (2) a polynucleotide operably linked to a different promoter which is activated by said ligand-dependent transcription factor, the polynucleotide encoding an IL-12 polypeptide at least 90% identical to IL-12, and one or and one or more immunomodulator polypeptides at least 90% identical to a protein selected from PD-LI and CTLA4.