发明名称 |
METHOD FOR THE DETECTION, PREPARATION AND DEPLETION OF CD4+ T LYMPHOCYTES |
摘要 |
The present invention relates an in vitro method for detecting class II restricted CD4+ T cells in a sample. Herein a sample is contacted with an isolated complex of an MHC class II molecule and a peptide. This peptide comprises an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif. CD4+ T cells are detected by measuring the binding of the complex with cells in the sample, wherein the binding of the complex to a cell is indicative for the presence of CD4+ T cells in the sample. The present invention further relates to an isolated complex of an MHC Class II molecule and a peptide comprising an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif. |
申请公布号 |
US2016091492(A1) |
申请公布日期 |
2016.03.31 |
申请号 |
US201414894221 |
申请日期 |
2014.05.27 |
申请人 |
IMCYSE SA |
发明人 |
SAINT-REMY Jean-Marie;CARLIER Vincent;VANDER ELST Luc |
分类号 |
G01N33/569;C07K17/14;C07K14/725;C07K14/74 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Liège BE |