| 摘要 |
Disclosed is a single immunoglobulin variable domain (dAb), which is a VH dAb which binds to TNFR1 and has an amino acid sequence that is, at least 80% identical to the DOM1H-131-206dAb amino acid sequence, which further comprises one or more modifications selected from: (a) a C-terminal extension which comprises an amino acid extension of from one amino acid to 5 amino acids; or (b) one or more amino acid framework substitutions wherein at least one substitution is a substitution selected from: a P14A substitution, a P41A substitution and a L108A substitution; or (c) a C terminal deletion, and which has reduced binding to pre-existing ADAs compared to the unmodified single immunoglobulin variable domain (dAb). |
