| 摘要 |
A method of producing an autologous composition for treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal is provided. The method comprises the steps of preparing an anti- inflammatory/ anti-catabolic component of the autologous composition comprising IL-1ra and TIMPs. The step of preparing anti-inflammatory/ anti-catabolic component comprising the following steps: collecting blood from the mammal; delivering the blood to a tube; incubating the blood at a temperature of from about 37°C to about 39°C for about 24 hours; centrifuging the blood to separate the blood into a supernatant component and a cellular fraction; collecting the supernatant component. The method further comprises the step of preparing a regenerative component of the autologous composition comprising the following steps: collecting blood from the mammal; delivering the blood to a tube in the presence of about 4% citric acid; centrifuging the blood to separate a platelet rich plasma component from a whole blood; collecting the platelet rich plasma component; and mixing the supernatant component with the platelet rich plasma component to provide the autologous composition. Also provided is a method of treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a patient with the autologous composition, an autologous composition for treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal and the use of the autologous composition for the treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal. |
