METHOD FOR TRANSFERRING PRECISE SMALL VOLUMES OF LIQUIDS

摘要

In a method for transferring precise small volumes of liquids (54, 64), the pressure in a defined volume portion (14) of a fluid passageway (12) is measured by means of two pressure sensors in fluid communication with the defined volume portion. A liquid (54, 64) is then added to the defined volume portion of the fluid passageway until the measured pressure indicates that a predetermined volume of liquid has been transferred into the passageway. The measured volume of liquid can then be transferred from the fluid passageway to a reactor (13). The invention has particular utility for chemical reactions carried out using complex manipulations (e.g. combinatorial chemistry) or the synthesis of complex compositions such as peptides. Using solid phase peptide synthesis (SPPS).