Cryogenic storage container, storage device, and methods of using the same

摘要

A device for the cryopreservation and/or vitrification of a biological sample in a freezing medium is described. The device preferably comprises a bath for receiving a vessel containing a biological sample in liquid-phase nitrogen. In one example, the bath includes a first member extending generally vertically from a bottom wall portion which divides the interior chamber of the bath into a first sub-chamber adapted for receiving a volume of cryogenic freezing medium and a second or overflow sub-chamber adapted to receive freezing medium from the first sub-chamber. A second member extends generally horizontally between the sidewall portions of the bath and includes at least one opening for receiving a biological sample-containing vessel(s). A kit including a cryogenic storage device and a vessel is also described.

主权项

1. A cryogenic storage device comprising:
a container comprising a bottom wall portion and at least two side wall portions, the bottom and side wall portions forming an interior chamber and wherein the container comprises an inner surface and an outer surface, a thermal insulating material covering at least a portion of one or both of the inner and outer surfaces of the container; a first member having a surface extending generally vertically from the bottom wall portion of the container and dividing the interior chamber into a first sub-chamber adapted for receiving a volume of cryogenic freezing medium and a second sub-chamber; a second member having a surface extending generally horizontally between the sidewall portions of the container into the first sub-chamber, wherein the generally horizontal surface comprises at least one opening formed therein configured to receive one or more biological sample-containing vessels.