Device and method for extracting target objects from a sample

摘要

The invention provides devices, systems, and methods for extracting target objects from a sample. In the method, a stream of sample containing a plurality of target and non-target objects is directed by first and second streams of buffer through a sample inlet channel into a fluid junction and through the fluid junction into a sample waste channel. In response to detecting a target object within the stream of sample, an actuator is energized to close a normally open valve, resulting in a transient burst of cross-flow into the fluid junction that briefly diverts the flow of sample within the fluid junction and results in an aliquot of sample being directed into an aliquot delivery channel. The combination of the valve and the actuator acts as a self-limiting pulse generator.

主权项

1. A microfluidic device for extracting target objects from a sample comprising:
an aliquotting segment comprising a sample inlet channel, a first buffer inlet channel, a sample waste channel, and an aliquot delivery channel fluidly coupled at a fluid junction, the sample inlet channel and first buffer inlet channel in fluid communication with an inlet end of the fluid junction and the sample waste channel and aliquot delivery channel in fluid communication with an outlet end of the fluid junction, the aliquotting segment further comprising a second buffer inlet channel and a normally open valve, the second buffer inlet channel coupled to the fluid junction between the sample inlet channel and the sample waste channel, and the normally open valve operably coupled to the second buffer inlet channel; and a trapping segment in fluid communication with the aliquotting segment, the trapping segment comprising a trapping chamber, a plurality of trapping structures, a collection channel, and a waste channel, wherein the trapping structures are disposed in the trapping chamber, and wherein the collection channel and the waste channel are in fluid communication with the trapping chamber.