| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to experimental medicine, general toxicology and nanotoxicology, and concerns criteria for diagnosis of toxic action of silver nanoparticles encapsulated in a natural polymer matrix of arabinogalactan (AG) on brain tissue of rats in long term. Method comprises exposing animal test group to toxicant by intragastric administration of an aqueous solution of AG with silver nanoparticles (nano-Ag-AG) in amount of 100 mcg per kilogram of silver in 0.5 ml of distilled water for 9 days. Control animals a subjected to intragastric administration of 0.5 ml of distilled water and for 9 days. After 6 months sectioning of cortex tissue from both groups of animals is performed. Stained with antibody to protein caspase-3, and survey microscopy of obtained preparations is performed. Number of pathologically altered neurons without expression of caspase 3, number of pathologically altered neurons expressing caspase 3, number of normal neurons without expression of caspase 3, number of normal neurons expressing caspase 3, total number of neurons per unit cut area is determined. Ratio of said indicators on preparations of experimental group to that of control group on preparations is calculated. By increasing number of pathologically altered neurons without expression of caspase 3 by 1.6 times relative to control group, increase in number of pathologically altered neurons expressing caspase 3 by 2.7 times relative to control group, reducing number of normal neurons without caspase 3 0.6 times in relation to control group, increase in number of normal neurons expressing caspase 3 2-fold relative to control group as well as reducing total number of neurons per unit area of 0.5 times relative to control group of development of inferring pathological process predominant damage type apoptosis in brain tissue in long term effects of nano-Ag-AG.EFFECT: method improves accuracy of verification toxic encephalopathies, including due to registration of functional changes of morphometric characteristics of neurons.1 cl, 1 tbl, 1 ex |
