Methods and Compositions for Highly Sensitive Detection of Molecules

摘要

Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

主权项

1. An analyzer comprising:
a) an electromagnetic radiation source for stimulating a fluorescent moiety; b) a capillary flow cell for passing said label; c) a source of motive force for moving said label in said capillary flow cell; d) an interrogation space defined within said capillary flow cell for receiving electromagnetic radiation emitted from said electromagnetic source; and e) an electromagnetic radiation detector operably connected to said interrogation space for measuring an electromagnetic characteristic of a stimulated fluorescent moiety; a processor operatively connected to the detector, wherein the processor is configured to execute instructions stored on a non-transitory computer-readable medium, and wherein the instructions, when executed by the processor, cause the processor to:
determine a threshold photon value corresponding to a background signal in the interrogation space, determine the presence of a fluorescent moiety in the interrogation space in each of a plurality of bins by identifying bins having a photon value greater than the threshold value, and compare the number of bins having a photon value greater than the threshold value to a standard curve.