METHODS AND COMPOSITIONS FOR TARGETED GENE MODIFICATION

摘要

Disclosed herein are methods and compositions for gene targeting utilizing fusion molecules comprising a recombinase domain and a sequence-specific DNA-binding domain.

主权项

1. A method of transfecting a cell comprising
introducing into the cell:
an exogenous nucleic acid and a nucleoprotein filament of a proteinaceous fusion molecule and a nucleic acid probe complementary to a target site of DNA of the cell,wherein the fusion protein comprises a recombinase domain that contributes to the filament, a DNA-binding domain, and a nuclear localization signal domain, wherein the exogenous nucleic acid is incorporated into the DNA of the cell and expressed by the cell.