NOVEL PEPTIDYL ALPHA-HYDROXYGLYCINE ALPHA-AMIDATING LYASES

摘要

The present patent application concerns an enzyme capable of catalysing the conversion of a α-hydroxyglycine to an α-amide and the use of such enzymes for producing a C-terminal α-amidated peptide.

主权项

1. An isolated polypeptide capable of catalysing the conversion of a α-hydroxyglycine to an α-amide, wherein said polypeptide comprises an amino acid sequence comprising the following motif 1:
Xaa1 Val Xaa2 Asp Arg Xaa3 Xaa4 Xaa5 Arg Xaa6 Gln Xaa7 Xaa8 Xaa9 Xaa10 Xaa11 Gly Xaa12 Xaa13 Xaa14 Xaa15 Xaa16 Trp; where Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, Xaa6, Xaa7, Xaa8, Xaa9, Xaa10, Xaa11 Xaa12, Xaa13. Xaa14, Xaa15, and Xaa16 are selected independently from a natural accurring amino acid, provided that Xaa1 and Xaa7 are not Cys.